Chromosome 3p Microsatellite Allelotyping in Neuroblastoma: A Report on the Technical Hurdles

Jasmien Hoebeeck Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

Bram De Wilde Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

Evi Michels Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

Valérie Combaret Molecular Oncology Unit, Centre Léon Bérard, Lyon, France

Nurten Yigit Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

Els De Smet Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

Nadine Van Roy Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

Eric Stanbridge Department of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA

Ning Ru Department of Microbiology and Molecular Genetics, University of California, Irvine, CA, USA

Geneviève Laureys Department of Pediatric Hematology and Oncology, Ghent University Hospital, Ghent, Belgium

Anne De Paepe Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

Frank Speleman Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

Jo Vandesompele Center for Medical Genetics, Ghent University Hospital, Ghent, Belgium

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Abstract

Pinpointing critical regions of recurrent loss may help localize tumor suppressor genes. To determine the regions of loss on chromosome 3p in neuroblastoma, we performed loss of heterozygosity analysis using 16 microsatellite markers in a series of 65 primary tumors and 29 neuroblastoma cell lines. In this study, we report the results and discuss the technical hurdles that we encountered during data generation and interpretation that are of relevance for current studies or tests employing microsatellites. To provide functional support for the implication of 3p tumor suppressor genes in this childhood malignancy, we performed a microcell-mediated chromosome 3 transfer in neuroblastoma cells.

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Chromosome 3p Microsatellite Allelotyping in Neuroblastoma: A Report on the Technical Hurdles

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Chromosome 3p Microsatellite Allelotyping in Neuroblastoma: A Report on the Technical Hurdles

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