M6A Regulatory Genes Play an Important Role in the Prognosis, Progression and Immune Microenvironment of Pancreatic Adenocarcinoma

Abstract

The specific roles of N6-methyladenosine (m6A) regulatory genes in pancreatic adenocarcinoma (PAAD) have not been fully elucidated. In present study, a novel risk signature was constructed by five m6A-related genes (including METTL3, METTL14, KIAA1429, ALKBH5 and YTHDF1) and was identified as an independent prognostic factor (HR = 13.192) via TCGA (185 samples) databases. The immune abundances of 22 leukocyte subtypes in each PAAD sample were exhibited via the CIBERSORT algorithm. High risk group promoted infiltration levels of Macrophages M0 and M2 cells and decreased that of B cells naive, T cells CD8 and T regulatory cells.

Keywords:

• Pancreatic adenocarcinoma
• N6-methyladenosine
• risk signature
• prognosis
• immune microenvironment

Acknowledgements

The authors thank Editage (https://www.editage.cn) for providing linguistic assistance. The first author also thank Mrs.Xin Wang for encouragement and support.

Consent for publication

The authors declare that they have consent for publication.

Code availability

The authors could provide the analytical code of Perl and R language for reasonable request.

Author contributions

Honghong Pei and Ling Bai conceived and designed the study. Fangshi Xu, Zhengliang Zhang, Miao Yuan and Yidi Zhao analyzed and interpreted the data. Fangshi Xu, Zhengliang Zhang, Miao Yuan and Yang Zhou wrote the manuscript. All authors participated in the revision of manuscript.

Declaration of interest

The authors declare that they have no competing interests.

Data availability statement

The datasets used and/or analyzed in the current study are available from the corresponding author upon reasonable request.