Circulating Long Non-Coding RNA GAS5: A Non-Invasive Molecular Marker for Prognosis, Response to Treatment and Survival in Non-Small Cell Lung Cancer

Abstract

Circulating long non-coding RNAs (lncRNA) are dysregulated in several diseases, especially cancers, e.g. non-small-cell lung cancer (NSCLC). Of specific notice in this regard is growth arrest-specific 5 gene (lncRNA GAS5), which is principally recognised as a tumor suppressor gene in numerous cancers. Functionally, GAS5 is involved in arresting cellular growth and induction of apoptosis. We analysed plasma GAS5 expression by qRT-PCR in 100 patients with NSCLC before and after tumour resection surgery. We reported a downregulation of GAS5 expression in NSCLC tissue and plasma, which showed elevation after surgery. Downregulation of GAS5 was associated with poor prognosis of NSCLC patients.

Keywords:

• Non-small-cell lung cancer
• qRT-PCR
• lncRNA-GAS5
• plasma
• tissues

Ethical approval

The study was approved by the Committees of Ethics of Research, Zagazig University, Egypt and East Jeddah Hospital, Jeddah, SA. Informed consent was obtained from all participating patients before enrollment in the study.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

Data availability statement

The datasets used and/or analysed during the current study are available from the corresponding author on request.

Additional information

Funding

The authors would like to extend their sincere thanks to Taif University Researchers Supporting Project Number [TURSP-2020/82], Taif University, Taif, Saudi Arabia for funding this research.