Myeloproliferative Neoplasms Transcriptome Reveals Pro-Inflammatory Signature and Enrichment in Peripheral Blood Monocyte-Related Genes

Abstract

Myeloproliferative neoplasms (MPN) are hematological diseases associated with genetic driver mutations in the JAK2, CALR, and MPL genes and exacerbated oncoinflammatory status. Analyzing public microarray data from polycythemia vera (n = 41), essential thrombocythemia (n = 21), and primary myelofibrosis (n = 9) patients’ peripheral blood by in silico approaches, we found that pro-inflammatory and monocyte-related genes were differentially expressed in MPN patients’ transcriptome. Genes related to cell activation, secretion of pro-inflammatory and pro-angiogenic mediators, activation of neutrophils and platelets, coagulation, and interferon pathway were upregulated in monocytes compared to controls. Together, our results suggest that molecular alterations in monocytes may contribute to oncoinflammation in MPN.

Keywords:

• Monocytes
• immune cells
• inflammation
• myeloproliferative neoplasms
• transcriptome

Authors’ contributions

VLB interpreted the data, designed the figures, and wrote the paper; RFMF performed the in silico analysis, interpreted the data, and designed the figures; KCRMF, FAC, VLB, and RFMF designed the study, discussed the results, and revised the manuscript.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Correction Statement

This article has been corrected with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

This study was financed by São Paulo Research Foundation (FAPESP; grants #2019/18013-8 and #2018/19714-7), National Council for Scientific and Technological Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES, grant #88887.369858/2019-00), and Center for Cell-based Therapy (CTC, Grant #2013/08135-2).