Bioreactor configurations for adventitious root culture: recent advances toward the commercial production of specialized metabolites

Abstract

In vitro plant cell and organ cultures are appealing alternatives to traditional methods of producing valuable specialized metabolites for use as: pharmaceuticals, food additives, cosmetics, perfumes, and agricultural chemicals. Cell cultures have been adopted for the production of specialized metabolites in certain plants. However, in certain other systems, adventitious roots are superior to cell suspension cultures as they are organized structures that accumulate high levels of specialized metabolites. The cultivation of adventitious roots has been investigated in various bioreactor systems, including: mechanically agitated, pneumatically agitated, and modified bioreactors. The main relevance and importance of this work are to develop a long-lasting industrial biotechnological technology as well as to improve the synthesis of these metabolites from the plant in vitro systems. These challenges are exacerbated by: the peculiarities of plant cell metabolism, the complexity of specialized metabolite pathways, the proper selection of bioreactor systems, and bioprocess optimization. This review’s major objective is to analyze several bioreactor types for the development of adventitious roots, as well as the advantages and disadvantages of each type of bioreactor, and to describe the strategies used to increase the synthesis of specialized metabolites. This review also emphasizes current advancements in the field, and successful instances of scaled-up cultures and the generation of specialized metabolites for commercial purposes are also covered.

GRAPHICAL ABSTRACT

Keywords:

• Adventitious roots
• airlift bioreactors
• bubble column bioreactors
• cell cultures
• organ cultures
• scale-up process
• specialized metabolites
• stirred tank bioreactors

Acknowledgments

Hosakatte Niranjana Murthy is thankful for the “Brain Pool” (BP) program Grant No. 2022H1D3A2A02056665.

Disclosure statement

No potential conflict of interest was reported by authors.

Additional information

Funding

This work was supported by the National Research Foundation of Korea (NRF) grant (MSIT) (No. NRF-2020R1A2C2102401), Korea and Technology Innovation Program, (Grant number P0018148) funded by the Ministry of Trade, Industry & Energy (MOTIE, Korea).