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Journal of Biomolecular Structure and Dynamics Volume 7, 1990 - Issue 5
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Original Articles

NMR Studies of the Interaction of Bleomycin with (dC-dG)3

Michael P. Gamcsik Department of Radiology and Radiological Science Division of NMR Research, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
,
Jerry D. Glickson Department of Radiology and Radiological Science Division of NMR Research, The Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA
&
Gerald Zon Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA, 94494, USA
Pages 1117-1133 | Received 03 Oct 1989, Published online: 21 May 2012
 
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Abstract

The interaction of bleomycin A2 and Zn(II)-bleomycin A2 with the oligonucleotide (dC-dG)3 has been monitored by nuclear magnetic resonance spectroscopy. Binding of the drug to the oligonucleotide is indicated by an upfield shift of the bithiazole proton resonances consistent with partial intercalation of this group between base pairs. The effect of temperature and ionic strength on the binding of both free bleomycin and the Zn(II) complex has been studied. Consistent with earlier studies on polynucleotides, the rate of exchange between the free drug and the drug-oligonucleotide complex is rapid on the 1H NMR chemical shift time scale. Binding of the oligonucleotide induced changes in resonances assigned to protons in the metal-binding region of Zn(II)-bleomycin. Intermolecular nuclear Overhauser effect enhancements between bleomycin and the oligonucleotide have not been detected.

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Related Research Data

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