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Research Articles

Dynamics and conformational propensities of staphylococcal CntA

, ORCID Icon, , , , , & show all
Pages 4923-4935 | Received 25 Feb 2020, Accepted 08 Jun 2020, Published online: 23 Jun 2020
 

Abstract

Enzymes use transition metals as co-factors for catalytic roles in biological processes. Notably, manganese, iron, cobalt, nickel, copper and zinc are abundantly used. Staphylococcus aureus, a commensal bacterium asymptomatically, lies on the human body causing variety of infections. S. aureus is equipped by advanced virulence-regulatory circuits of metal acquisition like Cnt that acquires metals at infection sites by utilizing a nicotianamine-like metallophore staphylopine. Despite significant growth in structural studies, how CntA of Cnt system transmits conformational signal upon staphylopine recognition remains elusive. Here, we analyzed the structural changes adopted by CntA during close-to-open transition by computational approaches. CntA uses a bi-domain architectural form of domain II which performed 37° rigid body rotation and 1.1 Å translation assisted by inter-domain hinge cluster residues. Important clustered communities were found regulating the conformational changes in CntA where communities 4 and 5 are found crucial. Besides open and close states, the fluctuating regions sampled two additional intermediate states which were considered close or open previously. CntA prefers fluctuating the non-conserved regions rather than conserved where domain II turned out to be rigid and maintains a stable fold. Overall, the CntA system is a potential target for structural biologist to hamper such conformational behaviors at family level.

Communicated by Ramaswamy H. Sarma

Availability of data and materials

We have made sure and have submitted all the relevant data.

Disclosure statement

The authors declare no conflict of interests.

Author’s contribution

ZA carried out analyses and writing. AA designed work scheme and involved in writing. MU carried out analyses. SM, WA and involved in writing and editing. SN, TM and FN involved in proof reading. 

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