Fluorescence anisotropy studies on the Hoechst 33258-DNA interaction: the solvent effect

Abstract

Fluorescence anisotropy method was applied to characterize the interactions of DNA minor groove binder Hoechst 33258 with different solvents without and in the presence of DNA. It is important to study the interaction of small molecules with DNA for the purpose of better understanding the mechanism of their action, as well as to design novel and more effective compounds. Spectroscopic study of the ligand in different binary mixed solvents containing DMSO, alcohols and buffer was carried out. Studies were performed without and in the presence of DNA. Fluorescence anisotropy studies reveal the characteristics of Hoechst 33258 in different mixed solvents. The results show the strong dependence of the anisotropy of Hoechst 33258 on solvent content, viscosity and intermolecular interactions.

Communicated by Ramaswamy H. Sarma

Keywords:

• Hoechst 33258
• ct-DNA
• dimethyl sulfoxide
• mixed solvents
• fluorescence anisotropy
• molecular interactions

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This work was supported by the RA MES State Committee of Science, in the frames of the research project № 18T-1D012.