Biological effects and molecular docking studies of Catechin 5-O-gallate: antioxidant, anticholinergics, antiepileptic and antidiabetic potentials

Abstract

Gallocatechin gallate is a form of catechin and an ester of gallocatechin and gallic acid. This is an epimer of the gallate epigallocatechin. In this study, the effect of this molecule, containing a biologically active group, was investigated in terms of important metabolic enzymes (carbonic anhydrase isoenzymes I and II (hCA I and II), achethylcholinesterase (AChE) and α-glycosidase (α-Gly) enzymes). The molecular docking method used to compare the biological activities of the Catechin 5-O-gallate molecule against enzymes was used. Afterwards, the ADME/T analysis was performed to investigate the drug availability of the Catechin 5-O-gallate molecule and the parameters obtained from ADME/T analysis were examined. Continuation of this study, for evaluating antioxidant and radical scavenging capacity Catechin 5-O-gallate, cupric ion (Cu²⁺) reduction capacity by CUPRAC method, Fe³⁺-Fe²⁺ reducing capacity, DPPH free radical clarifying (DPPH·), ABTS radical clarifying (ABTS^•+) were performed separately and during the study, trolox, α-tocopherol BHT and BHA were used as the reference antioxidant compound. Comparisons were applied with the four standard substances.

Communicated by Ramaswamy H. Sarma

Keywords:

• Carbonic anhydrase
• acetylcholinesterase
• α-glycosidase
• antioxidant activity
• Catechin 5-O-gallate

Acknowledgement

This research was made possible by TUBITAK ULAKBIM, High Performance and Grid Computing Center (TR-Grid e-Infrastructure).

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work is supported by the Scientific Research Project Fund of Sivas Cumhuriyet University under the project number RGD-020.