Bovine serum albumin interaction, molecular docking, anticancer and antimicrobial activities of Co(II) Schiff base complex derived from Nophen ligand

Abstract

In this report, synthesis, characterization, biological and molecular modeling studies of Nophen Schiff base [N,N-bis(2-hydroxy-1-naphthaldehyde)-o-phenylenediamine] and Co(II)-Nophen complex have been furnished. BSA binding affinities of the ligand and Co(II)-Nophen complex have been appraised by UV-visible, fluorescence and cyclic voltammetric techniques. Spectroscopic measurements indicate strong binding of the complex with BSA protein through static quenching mechanism with binding constant in the order of 10⁴ M⁻¹. The negative shift of the peak potential in cyclic voltammetry suggested an electrostatic interaction. Molecular docking analysis reveals significant binding affinity (-6.3 kcal/mol) of the complex towards BSA protein. It is amazing that the in vitro cytotoxicity of Co(II)-Nophen complex against A549 cell lines (Human lung carcinoma cells) has remarkable potentials with 29 ± 1.2 µM as IC₅₀ value. Comparing the biological activity towards microorganisms, Co(II)-Nophen complex show substantial response than the Nophen ligand.

Communicated by Ramaswamy H. Sarma

Highlights

• Synthesis and Characterization of Co(II)-Nophen complex derived from Nophen ligand

• Analytical, computational and spectral techniques are performed

• BSA binding and molecular modeling of ligand and Co(II)-Nophen complex are studied

• Antimicrobial activity and cytotoxicity of synthesized compounds are examined

• In vitro cytotoxicity against A549 cell lines is notable for Co(II)-Nophen complex

Keywords:

• A549 cell lines
• cytotoxicity
• BSA interaction
• antimicrobial activity
• molecular docking
• Nophen ligand

Disclosure statement

The authors declare no competing interests.

Additional information

Funding

The authors acknowledge the financial support from University Grants Commission, New Delhi, India (F.No.43-412/2014(SR)). A.M.I. Jayaseeli and J. Jeevitha Rani, extent their sincere thanks to the Management, Jayaraj Annapackiam College for Women, Periyakulam, India for providing research facilities under BSR and DST-FIST support equipments and the financial support under JACFRP.