Abstract
A revival interest has been given to natural products as sources of phytocompounds to be used as alternative treatment against infectious diseases. In this context, we aimed to investigate the antimicrobial potential of Ziziphus honey (ZH) against twelve clinical bacterial strains and several yeasts and molds using in vitro and computational approaches. The well-diffusion assay revealed that ZH was able to induce growth inhibition of most Gram-positive and Gram-negative bacteria. The high mean growth inhibition zone (mGIZ) was recorded in E. coli (Clinical strain, 217), S. aureus followed by E. coli ATCC 10536 (mGIZ values: 41.00 ± 1 mm, 40.67 ± 0.57 mm, and 34.67 ± 0.57 mm, respectively). The minimal bactericidal concentrations (MBCs) and minimal fungicidal concentration values (MFCs) from approximately 266.33 mg/mL to over 532.65 mg/mL. Molecular docking results revealed that the identified compounds maltose, 2-furoic acid, isopropyl ester, 2,4-imidazolidinedione, 5-(2-methylpropyl)-(S)- and 3,4,5-trihydroxytoluene, S-Methyl-L-Cysteine, 2-Furancarboxylic acid, L-Valine-N-ethoxycarbonyl, Hexanoic acid, 3,5,5-trimethyl-, Methyl-beta-D-thiogalactoside, gamma-Sitosterol, d-Mannose, 4-O-Methylmannose, 2,4-Imidazolidinedione, 5-(2-methylpropyl)- (S) were found to have good affinity for targeted receptor, respectively. Through a 100-ns dynamic simulation research, binding interactions and stability between promising phytochemicals and the active residues of the studied enzymes were confirmed. The ADMET profiling of all identified compounds revealed that most of them could be qualified as biologically active with good absorption and permeation. Overall, the results highlighted the efficiency of ZH against the tested clinical pathogenic strains. The antimicrobial potential and the potency displayed by the identified compounds could imply their further pharmacological applications.
Communicated by Ramaswamy H. Sarma
Acknowledgments
This research has been funded by Scientific Research Deanship at the University of Hail, Hail, Saudi Arabia through project number GR-22 009.
Author contributions
Conceptualization, M.S., N.B and W.S.H.; methodology, A.H., R.B., M.A.R., M.S.; software, R.H.L, A.J.S.; validation, Z.S., M.A.R.; formal analysis, Mo.A., M.S; investigation, A.H.; data curation, A.H., R.B., and E.N.; writing—original draft preparation, M.S., N.B., E.N., W.S.H., and R.B.; writing—review and editing, Mo.A., M.S., N.B., E.N., W.S.H., and R.B.; visualization, M.S.; supervision, W.S.H. All authors have read and agreed to the published version of the manuscript.
Disclosure statement
No potential conflict of interest was reported by the authors.
Geolocation information
Hail, Saudi Arabia.