149
Views
1
CrossRef citations to date
0
Altmetric
Research Articles

Screening of traditional medicinal plant extracts and compounds identifies a potent anti-leishmanial diarylheptanoid from Siphonochilus aethiopicus

, ORCID Icon, ORCID Icon, ORCID Icon, , , ORCID Icon, , & ORCID Icon show all
Pages 2449-2463 | Received 25 Jan 2023, Accepted 14 Apr 2023, Published online: 18 May 2023
 

Abstract

Available anti-leishmanial drugs are associated with toxic side effects, necessitating the search for safe and effective alternatives. This study is focused on identifying traditional medicinal plant natural products for anti-leishmanial potential and possible mechanism of action. Compounds S and T. cordifolia residual fraction (TC-5) presented the best anti-leishmanial activity (IC50: 0.446 and 1.028 mg/ml) against promastigotes at 48 h and less cytotoxicity to THP-1 macrophages. These test agents elicited increased expression of pro-inflammatory cytokines; TNFα and IL-12. In infected untreated macrophages, NO release was suppressed but was significantly (p < 0.05) increased in infected cells treated with compound S. Importantly, Compound S was found to interact with LdTopoIIdimer in silico, resulting in a likely reduced ability of nucleic acid (dsDNA)-remodelling and, as a result, parasite proliferation in vitro. Thereby, Compound S possesses anti-leishmanial activity and this effect occurs via a Th1-mediated pro-inflammatory response. An increase in NO release and its inhibitory effect on LdTopoII may also contribute to the anti-leishmanial effect of compound S. These results show the potential of this compound as a potential starting point for the discovery of novel anti-leishmanial leads.

Communicated by Ramaswamy H. Sarma

Authors’ contributions

S Singh and IE conceptualized the work, designed the experiments, wrote and edited the manuscript. EM designed and executed the experiments, performed the data interpretation and wrote the manuscript. EM, JS and RJ performed experiments. EM performed the Real Time, MTT, Infectivity and Parasitemia assays. JS performed the experiments related to cytokine analysis and nitric oxide production. RJ performed in silico interaction of Compound S with LdTopoIIdimer. AC and GDG helped in writing the manuscript. IE, EM, JS and RJ revised the manuscript. S Singh, JI, NI and RPP evaluated the work progress. All authors contributed to the article and approved the submitted version.

Data availability

The contributions presented in the study are included in the research article and further inquiries can be directed to the corresponding authors.

Disclosure statement

The authors have no competing interest to declare.

Additional information

Funding

This study was partly funded by the Africa-India Mobility Fund (AIMF-18-004) awarded by the African Academy of Sciences to Ezenyi I.C., and a CSIR Scientist Pool Officer (SRA) funding agency research grant (13(9160-A)2021-Pool) provided to Dr. Evanka Madan. JS is a recipient of the BioCare Women Scientist Fellowship from DBT. The work in Shailja Singh’s lab at Special Centre for Molecular Medicine (SCMM), JNU, is supported by IRHPA IPA/2020/000007, Department of Science and Technology and Drug and Pharmaceuticals Research Program (DPRP) (Project No. P/569/2016-1/TDT, SS). The work in Ramendra Pati Pandey’s lab is supported by Indian Council of Medical Research (ICMR) (Project No. 6/9-7(275) KA/2021/ECD-II). RJ acknowledges Research Associate I fellowship from Indo-DBT (Department of Biotechnology, Government of India) and Swiss National Science Foundation (SNSF). The funders had no role in the study design; collection, analysis, and interpretation of data; in the writing of the report; and in the decision to submit this article for publication.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.