Circadian fluctuations in circulating plasminogen activator inhibitor-1 are independent of feeding cycles in mice

ABSTRACT

To evaluate the involvement of the day-night feeding cycle in the circadian regulation of circulating plasminogen activator inhibitor-1 (PAI-1) concentrations, mice were fed with a diet for eight hours during either daytime (DF) or nighttime (NF) for one week. The reversed feeding cycle did not affect the circadian phases of plasma PAI-1 levels as well as the nocturnal wheel-running activity, although the phase of Pai-1 mRNA expression was significantly advanced for 8.6 hours in the livers of DF, compared with NF mice. The day-night feeding cycle is not a critical Zeitgeber for circadian rhythm of circulating PAI-1.

KEYWORDS:

• Circadian clocks
• circadian rhythm
• feeding behavior
• fibrinolysis
• mouse
• plasminogen activator inhibitor-1

Acknowledgments

We thank Sayaka Higo-Yamamoto (AIST) and Chiaki Hashimoto (AIST) for technical assistance.

Declaration of interest

The authors have no conflicts of interest to declare. The authors are solely responsible for the content and writing of this paper.

Funding

This study was supported by the Japan Society for 255 the Promotion of Science (JSPS) KAKENHI Grant Numbers JP16K00940 (to K. Oishi) and JP16K00920 (to N. Ohkura).

Additional information

Funding

This study was supported by the Japan Society for 255 the Promotion of Science (JSPS) KAKENHI Grant Numbers JP16K00940 (to K. Oishi) and JP16K00920 (to N. Ohkura)