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Chronobiology International
The Journal of Biological and Medical Rhythm Research
Volume 39, 2022 - Issue 5
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Original Article

DNA methylation of circadian genes and markers of cardiometabolic risk in female hospital workers: An exploratory study

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Pages 735-746 | Received 25 Oct 2021, Accepted 14 Jan 2022, Published online: 03 Feb 2022
 

ABSTRACT

Night shift work has been linked to increased risk of cardiovascular disease (CVD); however, the underlying mechanisms remain unclear. A compelling yet understudied mechanism involves differential DNA methylation of circadian genes. To investigate the relevance of this mechanism, we conducted an exploratory cross-sectional study of 74 female hospital personnel (38 day workers, 36 night shift workers). Sociodemographic, lifestyle, and health characteristics as well as shift work status and history were determined through self-report. Fasting blood samples were collected to measure markers of cardiometabolic risk and DNA was extracted to measure DNA methylation of 1150 cytosine-guanine (CpG) sites across 22 circadian genes. Associations between methylation levels at individual CpG sites (β-values) and markers of cardiometabolic risk were analyzed while considering effect modification by shift work status. The false discovery rate was applied to account for multiple comparisons (q ≤ 0.20). Two CpG sites [cg06758649 (CRY1) and cg06899802 (CSNK1A1)] were differentially associated with waist circumference and body mass index by shift work status, and eight CpG sites [cg26103512 (CSNK1D), cg03941313 (CSNK1E), cg18217763 (CSNK1E), cg16682686 (DEC1), cg12061096 (RORA), cg10133825 (RORA), cg19652148 (RORA), and cg22904654 (RORA)] were differentially associated with LDL cholesterol concentration by shift work status (all q ≤ 0.20). Our findings suggest that the relationship between DNA methylation of circadian genes and cardiometabolic risk differs by day and night shift worker status, which may contribute to mechanisms of increased risk of CVD observed among night shift workers.

Acknowledgements

We thank study participants at the Kingston Health Sciences Centre, study personnel, and Génome Québec for their laboratory and technical expertise in service of our data acquisition. This study was completed in partial fulfillment of the first author’s degree requirements for a Master of Science Epidemiology at Queen’s University.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

Financial support was received from the Canadian Institutes of Health Research (grant number 111023), the Cancer Research Society of Montréal (grant number 23218), and Queen’s University Faculty of Health Sciences Garfield-Kelly Grant.

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