Association between the Extent of Peripheral Blood DNA Methylation of HIF3A and Accumulation of Adiposity in community-dwelling Women: The Yakumo Study

ABSTRACT

Introduction

DNA methylation in the CpG sites of intron 1 of HIF3A is associated with body mass index (BMI). This cross-sectional study investigated correlations between DNA methylation of HIF3A and BMI or adiposity parameters in the Japanese population.

Method

DNA methylation of HIF3A was quantified via pyrosequencing.

Result

DNA methylation of HIF3A differed only in women; DNA methylation level at cg27146050 was associated with visceral adipose tissue thickness and correlated with BMI and percent (%) body fat after excluding smokers.

Conclusion

Peripheral blood DNA methylation at the CpG site (cg27146050) of HIF3A correlated with VAT thickness in Japanese women.

KEYWORDS:

• hypoxia-inducible factor 3A
• DNA methylation
• obesity
• visceral adipose tissue
• general population

Acknowledgments

We thank the participants and staff of the Health Examination Program for Residents of Yakumo, Hokkaido, Japan. We also appreciate bioRxiv for accepting this article as a preprint; the preprint doi is 10.1101/2020.11.30.403709.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

Supplementary material

Supplemental data for this article can be accessed online at https://doi.org/10.1080/07435800.2022.2121967

Additional information

Funding

This work was supported by the Japan Society for the Promotion of Science (JSPS) under Grants-in-Aid for Scientific Research under Grant numbers 26293144, 17K09139, 16H06277 and 20K10515.