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Original Articles

Nemertean ecdysteroids: Relationship to reproduction

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Pages 7-13 | Received 08 Jul 1991, Accepted 01 Nov 1991, Published online: 25 Feb 2011
 

Summary

Ecdysteroids in non-reproductive, reproductive, and post-reproductive specimens of two nemerteans, Paranemertes peregrina and Pantinonemertes californiensis, were studied using radioimmunoassay (RIA). Ecdysteroid concentrations increased during the reproductive season in females of both species. These data suggest the possibility that ecdysteroids are involved in reproduction. Study of ecdysteroid profiles using reverse phase high-performance liquid chromatography (RP-HPLC) followed by RIA showed that the types and relative amounts of different ecdysteroid metabolites were similar between mature male and female Pan. californiensis. In addition, [3H]-ecdysone was injected into mature male and female Pan. californiensis. The resulting RP-HPLC profiles indicated that both sexes metabolized [3H]-ecdysone to similar products. Both sexes produced polar and apolar ecdysteroid conjugates as the major metabolites of [3H]-ecdysone, with only traces of [3H]-20-hydroxyecdysone.

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