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Summary
Fertilizing capacity of spermatozoa collected from various storage sites in the male and female squids were examined by inseminated eggs in the presence or absence of gelatinous substances of the oviducal gland origin. Over 90% of the eggs were fertilized by the spermatozoa collected from the female's seminal receptacle regardless of the presence or absence of the gelatinous substance. Elevation of the chorion and the formation of perivitelline space was observed only in the jellied eggs. All jellyless eggs without perivitelline space died in 4 days after insemination. Spermatozoa collected from the spermatophore in the male's accessory gland could fertilize the majority of the eggs. On the other hand, those from the male's vas deferens could fertilize only 30–50% of both the jellied and jellyless eggs. It may be concluded from these experiments that the gelatinous substance of the oviducal gland origin has nothing to do with fertilization. However, it is essential for the elevation of the chorion and the formation of the perivitelline space. It may also be concluded that squid spermatozoa become fertile before they are transferred to the female's seminal receptacle.