Resveratrol Attenuates Ankylosing Spondylitis in Mice by Inhibiting the TLR4/NF-κB/NLRP3 Pathway and Regulating Gut Microbiota

ABSTRACT

Ankylosing spondylitis (AS) is an autoimmune disease associated with disturbed gut microbiota. Currently, the treatments and outcomes of AS are not satisfactory. It is reported that resveratrol (RES) is a major phytoalexin with anti-inflammatory, antibacterial and some other pharmacological effects. However, there are no studies on the role of RES in AS. Therefore, this study aimed to explore the effect and mechanism of RES on AS. Proteoglycan and complete freund’s adjuvant were used to conduct an AS mouse model, and then the AS mice were gavaged with RES (20 mg/kg and 50 mg/kg) daily for 4 weeks. Subsequently, the effect of RES on AS mice was assessed by detecting disease severity, inflammatory cytokines, NLRP3 inflammasome, TLR4/NF-κB pathway, intestinal mucosal barrier function, intestinal microbial barrier function. The assessment results indicated that RES could significantly relieve progression and severity of AS, inhibit the expression of pro-inflammatory cytokines (tumor necrosis factor-α, interleukin-6, interleukin-17A, interferon-γ), and promote the expression of anti-inflammatory cytokines (interleukin-4). RES intervention caused the inhibition of NLRP3 inflammasome and TLR4/NF-κB pathway. In terms of intestinal barrier function, experimental results found RES increased zonula occludens-1 and occludin expression, and additionally, changed the composition of the gut microbiota by increasing levels of Lactobacillus and Bifidobacterium and reducing levels of Enterococcus faecalis and Escherichia coli. Collectively, RES protects PG-induced AS mice by inhibiting inflammatory responses and TLR4/NF-κB/NLRP3 pathway, restoring intestinal mucosal barrier function, and regulating the composition of the gut microbiota. In other words, RES is a potential candidate for the treatment of AS.

KEYWORDS:

• Ankylosing spondylitis
• gut microbiota
• inflammatory response
• resveratrol
• TLR4/NF-κB/NLRP3 signaling

Disclosure statement

No potential conflict of interest was reported by the authors.

Author contributions

Ming-hui Ding and Peng-gang Xu contributed to study concept and design. Ying Wang and Jun-li Zhang contributed to data acquisition and statistical analysis. Bao-di Ren drafted the manuscript; All authors commented on versions of the manuscript and approved the final manuscript.

Additional information

Funding

This work was supported by Clinical Collaborative Innovation Project of Integrated Chinese and Western Medicine of Shaanxi Provincial Administration of Traditional Chinese Medicine “Optimization of Integrated Chinese and Western Medicine Diagnosis and Treatment Plan for Active Ankylosing Spondylitis” (No:2020-ZXY-006).