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Abstract
When Mycobacterium phlei is subjected to sonication, a solid and a water soluble fraction are obtained. The water soluble material is highly surface-active and adsorbs onto both dolomite and apatite. In both cases the mineral becomes more hydrophobic with the effect being greater on dolomite than on apatite. The change in surface characteristics of the minerals was monitored by contact angle, zeta potential and micro flotation experimentation. The data obtained were compared to results obtained when whole cells of M.phlei were used in place of the soluble fraction. Implications on the effect of the addition of both M.phlei and its soluble fraction on the flotation separation of the two minerals are discussed.
