Establishing an animal model for peritoneal catheter malfunction caused by omental wrapping using negative pressure suction: in vitro and in vivo exploration

Abstract

Background

This study aims to establish a simplified and effective animal model of catheter malfunction caused by omental wrapped using negative pressure suction.

Method

The peritoneal dialysis catheter outlet was linked to a negative-pressure (0-75mmHg) suction pump to intensify the negative pressure. Different negative pressures were tested for model construction in vitro. In vivo, a model of peritoneal catheter malfunction caused by omental wrapped was constructed in five beagles after catheter placement. Catheter drainage conditions and related complications were monitored before and after the model establishment.

Results

In the vitro experiment, the overall success rate of constructed models was 90% (36/40). The total malfunction rate was higher in 62.5 mmHg (10/10) and 75 mmHg (10/10) than in 12.5 mmHg (8/10) and 37.5 mmHg (8/10). The outflow velocity of dialysate at 62.5 mmHg was significantly lower than that at 12.5 mmHg and 37.5 mmHg, without a statistically significant difference compared to 75 mmHg. In the in vivo experiment, catheter outflow velocity increased, and residual fluid volume decreased after omental wrapped (99.6 ± 6.7 ml/min vs. 32.6 ± 4.6 ml/min at initial five minutes, p < 0.0001; 69.2 ± 16.3 ml vs. 581.0 ± 109.4 ml, p < 0.001). And the outflow velocity was finally below 2 ml/min. No severe related complications (such as infection, organ damage, or bleeding) were observed through laparoscopic examination and dialysate tests seven days post-operation.

Conclusion

Utilizing negative pressure suction to increase negative pressure around catheter tip is a simple, safe, and effective method for establishing an animal model of omental wrapped leading to catheter malfunction.

Keywords:

• Peritoneal dialysis
• omental wrapped
• catheter malfunction
• catheter-related mechanical complication
• animal model

Acknowledgements

The authors are grateful to Dr. Laien Xue for technical support in animal experiment.

Ethical approval

The animal experiment protocols were approved by the Ethics Committee of 900th Hospital of Joint Logistics Support Force (IRB approval number 2022-014).

Authors’ contributions

YG, XW, WW contributed to the study conception and design. Material preparation and animal experiment were performed by XW, LY, JC, QS, MQ, QW, JY. Data collection, and analysis were performed by XW, LY, JC, QS. The first draft of the manuscript was written by XW. WW, LY, JC performed the statistical analysis. All authors read and approved the final manuscript. All authors searched the literature.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Data availability statement

The datasets used and analyzed during the current study available from the corresponding author on reasonable request.

Additional information

Funding

This study was supported by Guiding Project of Fujian Provincial Science and Technology Department (No. 2021Y0059), the Fujian Clinical Medical Research Center for Immune Kidney Disease and Fujian Scientific Innovation Joint Clinical Medical Research Center for Immune Kidney Disease and Fujian Scientific Innovation Joint Funding (2020Y9042 to WW).