Gene expression profiles reveal homeostatic dynamics during interferon-β therapy in multiple sclerosis

Viviana Annibali Department of Neurology and Center for Experimental Neurological Therapy, S. Andrea Hospital, University of Rome “La Sapienza”, Rome, Italy

Simone Di Giovanni Department of Neurology and Center for Experimental Neurological Therapy, S. Andrea Hospital, University of Rome “La Sapienza”, Rome, Italy

Stefania Cannoni Department of Neurology and Center for Experimental Neurological Therapy, S. Andrea Hospital, University of Rome “La Sapienza”, Rome, Italy

Elisabetta Giugni Neuroradiology, S. Andrea Hospital, University of Rome “La Sapienza”, Rome, Italy

Roberto Bomprezzi Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD20892, USA

Carlo Mattei Department of Neurology and Center for Experimental Neurological Therapy, S. Andrea Hospital, University of Rome “La Sapienza”, Rome, Italy

Abdel Elkahloun Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD20892, USA

Eliana Marina Coccia Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy

Marco Alfò Department of Statistics, Probability and Applied Statistics, University of Rome “La Sapienza”, Rome, Italy

Francesco Orzi Department of Neurology and Center for Experimental Neurological Therapy, S. Andrea Hospital, University of Rome “La Sapienza”, Rome, Italy

Giovanni Ristori Department of Neurology and Center for Experimental Neurological Therapy, S. Andrea Hospital, University of Rome “La Sapienza”, Rome, Italy

Marco Salvetti Department of Neurology and Center for Experimental Neurological Therapy, S. Andrea Hospital, University of Rome “La Sapienza”, Rome, Italy

show all

Abstract

Understanding the mechanisms that sustain the effects of disease modifying drugs in multiple sclerosis (MS) may help refine current therapies and improve our knowledge of disease pathogenesis.

By using cDNA microarrays, we investigated gene expression in the peripheral blood mononuclear cells (PBMC) of 7 MS patients, at baseline (T0) as well as after 1 (T1) and 3 months (T3) of interferon beta-1a (IFN-β-1a; Rebif™ 44 μg) therapy.

Gene expression changes involved genes of both immunological and non-immunological significance. We validated IL-10 up-regulation, which is in accordance with previous reports, and other novel changes that underscore the capacity of IFN-β to impair antigen presentation and migration of inflammatory elements into the central nervous system (up-regulation of filamin B and down-regulation of IL-16 and rab7).

Overall, gene expression changes became less pronounced after 3 months of therapy, suggesting a homeostatic response to IFN-β. This may be of use for the design of new treatment schedules.

Keywords

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Gene expression profiles reveal homeostatic dynamics during interferon-β therapy in multiple sclerosis

Information for

Open access

Opportunities

Help and information

Gene expression profiles reveal homeostatic dynamics during interferon-β therapy in multiple sclerosis

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature