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Original

Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies

Sandra A. Sciascia Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Kristina Robson Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Sandra A. Sciascia Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Kristina Robson Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Liangjin Zhu Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Sandra A. Sciascia Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Kristina Robson Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Liangjin Zhu Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Michael Garland Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Shannon Grabosch Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Joseph Kelamis Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Will Messamore Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Todd Bradley Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Allison Sourk Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Lindsey Westberg Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Waco Goodnight Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Krystina Tongson Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Naomi Holloway Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Zabi Wardak Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Michelle Sudyka Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Shriti Masrani Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Sanket Chintalapati Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Manuel Cagaanan Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

John C. Brown Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

Dean A. Stetler Department of Molecular Biosciences, University of Kansas, Lawrence, KS66045, USA

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Abstract

Antibodies against the N-terminal (NT) but not the basic domain (BD), DNA binding regions of the largest subunit (S1) of RNA polymerase I (RNAPI) were detected in the sera of MRL-lpr/lpr lupus mice. Antibodies against both RNAPI(S1)-NT and -BD, as well as other systemic lupus erythematosus (SLE) autoantigens (La, ribosomal P proteins and Sm/RNP) were produced by rabbits immunized with anti-DNA antibodies that had been affinity purified from SLE patients. Immunization of nonautoimmune mice (Balb/c) with RNAPI(S1)-NT, RNAPI(S1)-BD, or La in the form of GST fusion proteins, induced production of anti-double-stranded (ds) DNA and anti-Sm/RNP. GST-P1 did not induce an anti-dsDNA response in these mice. These results demonstrate that RNAPI(S1)-NT, RNAPI(S1)-BD and La can participate in an anti-autoantigen/anti-DNA antibody loop during an SLE-like autoimmune response.

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Acknowledgements

This work was funded by the Francis V. Stevens Memorial fund (KUEA).

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Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies

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Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies

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