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Abstract
The pain-relieving effects of low-dose radon therapies on patients suffering from chronic painful inflammatory diseases have been described for centuries. Even though it has been suggested that low doses of radiation may attenuate chronic inflammation, the underlying mechanisms remain elusive. Thus, the RAD-ON01 study was initiated to examine the effects of radon spa therapy and its low doses of alpha radiation on the human immune system. In addition to an evaluation of pain parameters, blood was drawn from 100 patients suffering from chronic painful degenerative musculoskeletal diseases before as well as 6, 12, 18, and 30 weeks after the start of therapy. We verified significant long-term pain reduction for the majority of patients which was accompanied by modulations of the peripheral immune cells. Detailed immune monitoring was performed using a multicolor flow cytometry-based whole blood assay. After therapy, the major immune cells were only marginally affected. Nevertheless, a small but long-lasting increase in T cells and monocytes was observed. Moreover, neutrophils, eosinophils and, in particular, dendritic cells were temporarily modulated after therapy. Regarding the immune cell subsets, cytotoxic T and NK cells, in particular, were altered. However, the most prominent effects were identified in a strong reduction of the activation marker CD69 on T, B, and NK cells. Simultaneously, the percentage of HLA-DR+ T cells was elevated after therapy. The RAD-ON01 study showed for the first time a modulation of the peripheral immune cells following standard radon spa therapy. These modulations are in line with attenuation of inflammation.
Acknowledgements
We would like to express our thanks to Beckman Coulter GmbH (Krefeld, Germany) for providing us with the TQ-Prep Workstation for the study. We also thank Dr. Nina Werthmöller, Dr. Yvonne Rubner, Renate Sieber, and Kathrin Köhn for their technical help in performing the immune monitoring for this study.
Declaration of interest
The authors have nothing to declare. This work was supported by the German Federal Ministry of Education and Research (BMBF) under Grant 02NUK017G (GREWIS), in part by the European Commission (European Network of Excellence, DoReMi) under Grant FP7-249689, by Landesamt für Gesundheit und Lebensmittelsicherheit Bayern (LGL), and by Bayerisches Staatsbad Bad Steben GmbH.