Abstract
Background
Circular RNAs (circRNAs) have critical roles in various types of diseases, including preeclampsia (PE). Circ_0005714 function in PE was explored in this study.
Methods
Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed for level analysis of circ_0005714, micoRNA-223-3p (miR-223-3p), and a disintegrin and metalloproteinase 9 (ADAM9). Cell Counting Kit-8 (CCK-8) and colony formation assays were used for cell viability and colony formation detection. Cell proliferation was determined by EdU assay. The determination of migration and invasion was conducted by wound healing assay and transwell assay. Tube formation assay was applied to assess angiopoiesis. Target binding analysis was performed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. Western blot was used for protein examination.
Results
Circ_0005714 was highly expressed in PE placenta tissues. The expression promotion of circ_0005714 reduced proliferation, migration, invasion, and angiopoiesis in trophoblast cells. Furthermore, circ_0005714 acted as a molecular sponge for miR-223-3p and the effects of circ_0005714 on trophoblast cells were achieved by sponging miR-223-3p. Moreover, miR-223-3p could target ADAM9 and knockdown of ADAM9 reversed cell progression inhibition induced by miR-223-3p inhibitor. In addition, circ_0005714 upregulated the ADAM9 expression and inactivated the Wnt/β-catenin pathway through targeting miR-223-3p.
Conclusions
All results manifested that circ_0005714 retarded the progression of PE by mediating the miR-223-3p/ADAM9 signal network.
Acknowledgements
None.
Disclosure statement
The authors declare that they have no conflicts of interest.
Authors’ contribution
Xuemei Hu was responsible for drafting the manuscript. Xuemei Hu and Weilan Xia contributed to the analysis and interpretation of data. Xuemei Hu and Weilan Xi contributed in the data collection. All authors read and approved the final manuscript.
Funding
None.
Availability of data and materials
Please contact the correspondence author for the data request.