Hochu-ekki-to combined with interferon-gamma moderately enhances daily activity of chronic fatigue syndrome mice by increasing NK cell activity, but not neuroprotection

Abstract

The purpose of this study was to evaluate the beneficial effect of Hochu-ekki-to (TJ-41) combined with interferon-gamma (IFNγ) on daily activity, immunological and neurological alternation in a mouse model of chronic fatigue syndrome (CFS). CFS was induced by 6 times of repeated injection of Brucella abortus antigen every 2 weeks. Both single TJ-41 and TJ-41 combined with IFNγ increased running activity and thymus weight of CFS mice, while thicker thymic cortex together with elevation of natural killer cell activity was only found in the combined treatment group. No significant improvement was observed in the atrophic brain and decreased expression level of brain-derived neurotrophic factor and Bcl-2 mRNA in hippocampus in both treatment groups. Our results suggest that TJ-41 combined with IFNγ might have a protective effect on the marked reduction in the activity in a model of CFS via normalization of host immune responses, but not neuroprotection.

Keywords::

• Chronic fatigue syndrome
• natural killer cell activity
• brain-derived neurotrophic factor (BDNF)
• B cell lymphoma-2 (Bcl-2)
• interferon-gamma
• Hochu-ekki-to (TJ-41)

Acknowledgments

This study was supported by a grant from the research fund of Institute of Kampo Medicine (Japan) (to Dr. Takahashi, in 2006), and a research grant from Grant-in-Aid for Scientific Research (B), the Ministry of Education, Science and Culture of Japan (No. 20790479, to Dr. J. Moriya, in 2008–10).

Declaration of interest: The authors report no conflicts of interest.