Combinatorial materials research applied to the development of new surface coatings V. Application of a spinning water-jet for the semi-high throughput assessment of the attachment strength of marine fouling algae

Abstract

In order to facilitate a semi-high throughput approach to the evaluation of novel fouling-release coatings, a ‘spinjet’ apparatus has been constructed. The apparatus delivers a jet of water of controlled, variable pressure into the wells of 24-well plates in order to facilitate measurement of the strength of adhesion of algae growing on the base of the wells. Two algae, namely, sporelings (young plants) of the green macroalga Ulva and a diatom (Navicula), were selected as test organisms because of their opposing responses to silicone fouling-release coatings. The percentage removal of algal biofilm was positively correlated with the impact pressure for both organisms growing on all the coating types. Ulva sporelings were removed from silicone elastomers at low impact pressures in contrast to Navicula cells which were strongly attached to this type of coating. The data obtained for the 24-well plates correlated with those obtained for the same coatings applied to microscope slides. The data show that the 24-well plate format is suitable for semi-high throughput screening of the adhesion strength of algae.

Keywords:

• Alga
• diatom
• fouling release
• high-throughput screen
• silicone elastomer
• Ulva
• Navicula

Acknowledgements

This study was carried out with support from the US Office of Naval Research in the form of grants N00014-03-1-0509 to JAC & MEC and N00014-02-1-0794 and N00014-03-1-0702 to NDSU.