![Sample our Physical Sciences journals, sign in here to start your FREE access for 14 days]({"type":"image" , "src" : "/sda/110819/TOC-Subject-Sample-2021-Physical-Sciences.jpg"})
Abstract
The protein resistance of dextran and dextran-poly(ethylene glycol) (PEG) copolymer films was examined on an organosilica particle-based assay support. Comb-branched dextran-PEG copolymer films were synthesized in a two step process using the organosilica particle as a solid synthetic support. Particles modified with increasing amounts (0.1–1.2 mg m−2) of three molecular weights (10,000, 66,900, 400,000 g mol−1) of dextran were found to form relatively poor protein-resistant films compared to dextran-PEG copolymers and previously studied PEG films. The efficacy of the antifouling polymer films was found to be dependent on the grafted amount and its composition, with PEG layers being the most efficient, followed by dextran-PEG copolymers, and dextran alone being the least efficient. Immunoglobulin gamma (IgG) adsorption decreased from ∼5 to 0.5 mg m−2 with increasing amounts of grafted dextran, but bovine serum albumin (BSA) adsorption increased above monolayer coverage (∼2 mg m−2) indicating ternary adsorption of the smaller protein within the dextran layer.
Acknowledgements
This project was financially supported by the Queensland Smart State National and International Research Alliances Program (RM2007001266) and the National Institutes of Health (U01 AI082186-01). The authors would like to thank Dr Barry Wood for his assistance with the XPS measurements and James Bates for the synthesis of the organosilica particles.