Biofilm inhibitors targeting the outer membrane protein A of Pasteurella multocida in swine

Abstract

Pasteurella multocida (Pm) is the causative agent of atrophic rhinitis in swine. This study aimed to discover biofilm inhibitors against swine Pm to counteract antibiotic resistance and decrease virulence. The virulence factor outer membrane protein A (OmpA) was targeted. A library of drugs approved by the Food and Drug Administration (FDA) was used to perform virtual screening against PmOmpA. The top-scoring compounds had no effect on the growth of Pm serotype A or D. Mycophenolate mofetil showed the highest efficacy in inhibiting biofilm formation by Pm serotype A, with an IC₅₀ of 7.3 nM. For Pm serotype D, indocyanine green showed the highest effect at an IC₅₀ of 11.7 nM. Nevertheless, these compounds had no effect on an established biofilm of Pm. This study offers an alternative way to prevent biofilm formation by Pm that could also be applied to other pathogens.

Keywords:

• Pasteurella multocida
• outer membrane protein A
• biofilm formation
• FDA-approved drugs
• virtual screening

Acknowledgements

The authors would like to thank Dr Teerasak E-kobon who kindly provided Pm serotypes A and D.