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Biofouling
The Journal of Bioadhesion and Biofilm Research
Volume 38, 2022 - Issue 8
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Research Articles

Biofouling of reverse osmosis membranes: assessment by surface-enhanced Raman spectroscopy and microscopic imaging

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Pages 852-864 | Received 23 Mar 2022, Accepted 19 Oct 2022, Published online: 31 Oct 2022
 

Abstract

The decline in the performance of spiral-wound reverse osmosis (SWRO) membranes is frequently due to biofouling. This study focus on qualitative and quantitative diagnosis of SWRO membrane biofouling. Bacterial counts on the different surfaces of the fouled membranes were carried out. Surface enhanced Raman spectroscopy (SERS) was performed to highlight clogging materials as well as their natures and identity. The topography of the fouled membranes and the structures of biofilms were visualized by fluorescence microscopy (FM) and scanning electron microscopy (SEM). The results indicated the presence of bacteria in the different SWRO membrane areas. Those strongly adhered were significantly higher than those weakly. It varied between 26 × 105 and 262 × 105 CFU m−2. However, SERS mapping showed different fouling levels and the thickness of the fouling layer was 5 µm. Microscopic imaging revealed biotic and abiotic deposits. These data can together allow better management of the seawater desalination process.

Disclosure statement

No potential conflict of interest was reported by the authors.

Acknowledgements

The authors wish also to thank Dayri Ahmed, director of the Souk T'lata seawater desalination plant, and Guermat Ismail, technical engineer, for their help and assistance.

Additional information

Funding

The authors are very thankful to the DGRSDT-MESRS - Algeria for their financial support.

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