Bacterial sulfidogenic community from the surface of technogenic materials in vitro: composition and biofilm formation

Abstract

Microbial biofilms of sulfate-reducing bacteria Desulfovibrio oryzae SRB1 and SRB2 were evaluated on polyethylene terephthalate in mono- and associative bacterial cultures. Bacillus velesensis strains C1 and C2b suppressed both the formation of biofilm and reduced the number of sulfate-reducing bacteria in the biofilm on the polyethylene terephthalate during the 50-day experiment. A decrease in the number of sulfate-reducing bacteria compared to the monoculture was also noted in association of D. oryzae SRB1 + Sat1 (bacterium-satellite of the sulfate-reducing bacteria). The strain Sat1 was identified as Anaerotignum (Clostridium) propionicum based on some microbiological, physiological and biochemical, genetic features. The importance of studying existing interactions between microorganisms in the ferrosphere and plastisphere is emphasized.

Keywords:

• Anaerotignum propionicum
• Bacillus velezensis
• biofilm
• Desulfovibrio oryzae
• polyethylene terephthalate
• qPCR

Acknowledgements

We are sincerely grateful to Demchenko N.R. for her help in the ultrasonic removal of the biofilm from the PET surface and Strekalov V.M. for electronic microscopy.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

The author(s) reported there is no funding associated with the work featured in this article.