Purification and characterization of a dopa‐containing protein from the foot of the Asian freshwater mussel, Limnoperna fortunei

Kousaku Ohkawa Institute of High Polymer Research, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386–8567, Japan

Ayako Nishida Institute of High Polymer Research, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386–8567, Japan

Kengo Ichimiya Institute of High Polymer Research, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386–8567, Japan

Yoshihiko Matsui Department of Civil Engineering, Gifu University, Yanagido, Gifu, 501–1193, Japan

Keiji Nagaya Department of Civil Engineering, Gifu University, Yanagido, Gifu, 501–1193, Japan

Akira Yuasa Center for River Basin Environmental Research, Gifu University, Yanagido, Gifu, 501–1193, Japan

Hiroyuki Yamamoto Institute of High Polymer Research, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386–8567, Japan Fax: E-mail: [email protected]‐u.ac.jp

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A Dopa‐containing protein was purified from the foot of the Asian freshwater mussel, Limnoperna fortunei, through acid‐urea extraction, 36% ammonium sulfate precipitation, Sephacryl S‐200 gel filtration, and reversed phase high performance liquid chromatogra‐phy. The apparent molecular mass of the protein was 96 kDa, and Achromobacter protease I digested this into six major fragments. The amino acid sequences of the fragments were determined as two hexapeptides and four decapeptides. The consensus sequence of the decapeptides was Lys‐(Hyp/Pro)‐Thr‐(Gln/Tyr)‐Dopa‐(Ser/Thr)‐(Asp/Thr)‐Glu‐Tyr‐Lys. These results suggest that the Dopa‐containing foot protein mostly consists of repetitive decapeptides and hexapeptides. The Dopa‐containing 96 kDa protein is considered to be a byssal precursor in L. fortunei.

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Corresponding author; fax: 81–268–21–5571; e‐mail: [email protected]‐u.ac.jp

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Purification and characterization of a dopa‐containing protein from the foot of the Asian freshwater mussel, Limnoperna fortunei

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Purification and characterization of a dopa‐containing protein from the foot of the Asian freshwater mussel, Limnoperna fortunei

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