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High Pressure Research
An International Journal
Volume 19, 2000 - Issue 1-6
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Original Articles

Pressure-induced activation and pressure-tolerance of mutated thermolysin at 119

, , , &
Pages 241-245 | Received 09 Sep 1999, Published online: 19 Aug 2006
 

Abstract

The pressure activation of thermolysin substitution at the 119th site was studied for four mutants and the wild type (Q119Q. Q119N, Q119R, Q119E and Q119D). The highest activation recorded over 30 fold and the activation volumes (ΔV‡) were about -75ml/mol for Q119Q, Q119N and Q119R, while Q119E and Q119D showed only a10 fold activation and ΔV‡ of around -6Oml/mol. The pressure-tolerance of these enzymes were investigated through the in situ observation of their intrinsic fluorescence. Q119E and Q119D showed smaller ΔG app and ΔG app of transition than the wild type.

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