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High Pressure Research
An International Journal
Volume 19, 2000 - Issue 1-6
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Original Articles

Structural prerequisites for the stability of Sso7d from the archaeon Sulfolobus solfataricus versus high pressure and temperature

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Pages 311-316 | Received 09 Sep 1999, Published online: 19 Aug 2006
 

Abstract

Sso7d is a protein purified from the thermoaddophilic archaeon Sulfolobus solfataricus. In this paper we present the effect of pressure and temperature on the stability of Sso7d and its mutants. Fourth derivative UV spectroscopy and ANS (I-anilinonaphtalene-8-sulfonate) binding were used to monitor the protein unfolding.

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