Structural prerequisites for the stability of Sso7d from the archaeon Sulfolobus solfataricus versus high pressure and temperature

Enrico Mombelli INSERM U128 (IFR24) 1919 Route de Mende, F-34293, Montpellier Cedex 5, France

Erlet Shehi Dipartimento di Fisologia e Biochimica Generali, Università di Milano, via Celoria 26, 20133, Milano, Italy

Paola Fusi Dipartimento di Fisologia e Biochimica Generali, Università di Milano, via Celoria 26, 20133, Milano, Italy

Paolo Tortora Dipartimento di Fisologia e Biochimica Generali, Università di Milano, via Celoria 26, 20133, Milano, Italy

Reinhard Lange INSERM U128 (IFR24) 1919 Route de Mende, F-34293, Montpellier Cedex 5, France

Claude Balny INSERM U128 (IFR24) 1919 Route de Mende, F-34293, Montpellier Cedex 5, France

Abstract

Sso7d is a protein purified from the thermoaddophilic archaeon Sulfolobus solfataricus. In this paper we present the effect of pressure and temperature on the stability of Sso7d and its mutants. Fourth derivative UV spectroscopy and ANS (I-anilinonaphtalene-8-sulfonate) binding were used to monitor the protein unfolding.

Key Words:

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Structural prerequisites for the stability of Sso7d from the archaeon Sulfolobus solfataricus versus high pressure and temperature

Information for

Open access

Opportunities

Help and information

Structural prerequisites for the stability of Sso7d from the archaeon Sulfolobus solfataricus versus high pressure and temperature

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature