Publication Cover
High Pressure Research
An International Journal
Volume 30, 2010 - Issue 4
56
Views
2
CrossRef citations to date
0
Altmetric
Selected papers from the 6th International Conference on High Pressure Bioscience and Biotechnology (HPBB 2010) in Freising (Germany)

Activation volume of crystallization and effects of pressure on the three-dimensional nucleation rate of glucose isomerase

, &
Pages 483-489 | Received 30 Aug 2010, Accepted 21 Sep 2010, Published online: 02 Nov 2010
 

Abstract

The activation volume, Δ V , of the solute incorporation process at the kink site of a glucose isomerase (GI) crystal was estimated using the pressure dependency of the step kinetic coefficient, βstep, and the three-dimensional nucleation rates J of GI crystals were analyzed using classical nucleation theory. Δ V took a negative value. The sticking parameter, s, for the addition of a GI tetramer to a critical nucleus drastically increased with increasing pressure. This was probably due to the decrease in the activation energy needed for the addition of the GI tetramer.

Acknowledgements

This work was partially supported by Grants-in-Aid (Nos. 16760014 and 19760009 (Y.S.)) for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.