Man-Made Mineral Fiber Hazardous Properties Assessment Using Transgenic Rodents: Example of Glass Fiber Testing

Abstract

Transgenic BigBlue rats were exposed to CM 44 glass fibers (6.3 mg/m 3) by nose only, 6 h/day for 5 days. Two endpoints were examined 1, 3, 14, 28, and 90 days following exposure: fiber biopersistence and mutations in lung DNA. The half-time of the fibers >20 µm was 12.8 days, and mutant frequencies of control and exposed rats were similar across all time points. The mutation spectra of both series were similar after 28 days of fixation time. These results showed that a glass fiber with a high clearance in the lung seems to not present any significant effect on mutagenesis on lung DNA and are in marked contrast to results for asbestos, which caused a twofold mutant frequency increase as described in a previous study (Rihn et al., 2000b).