Human brain endothelial cells (HUBEC) promote SCID repopulating cell expansion through direct contact

Xiangfei Cheng Greenebaum Cancer Center, University of Maryland, 655 W. Baltimore Street, Baltimore, MD, 21201, USA

Thomas Macvittie Greenebaum Cancer Center, University of Maryland, 655 W. Baltimore Street, Baltimore, MD, 21201, USA

Barry Meisenberg Greenebaum Cancer Center, University of Maryland, 655 W. Baltimore Street, Baltimore, MD, 21201, USA

Emily Welty Greenebaum Cancer Center, University of Maryland, 655 W. Baltimore Street, Baltimore, MD, 21201, USA

Ann Farese Greenebaum Cancer Center, University of Maryland, 655 W. Baltimore Street, Baltimore, MD, 21201, USA

Douglas Tadaki Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD, 20910, USA

Naoko Takebe Greenebaum Cancer Center, University of Maryland, 655 W. Baltimore Street, Baltimore, MD, 21201, USACorrespondence[email protected]

show all

Abstract

The objective of this study was to re-evaluate the previously published hematopoietic stem cell (HSC) expansion work using human brain endothelial cells (HUBEC). The expansion effect of contact and non-contact conditions was reported to be equivalent by others. However, we report here different results that the expansion can be achieved only with direct contact. We co-cultured human CD34+ cells with and without HUBEC contact for seven days with cytokines and the readouts were CD34+/CD38 − phenotype and SCID repopulating cell (SRC) frequency. Also tested was the inhibitory effect of Wnt receptor inhibitor Dkk-1 on HUBEC contact ex vivo expansion; whether an increased expression of Wnt3 occurs on the HUBEC surface; and detection of an increased nuclear localization of beta-catenin in CD34+/CD38 − cells in HUBEC contact culture condition. We conclude that the successful expansion by HUBEC contact culture is a candidate explanation based on the Wnt family protein, possibly Wnt3, expression on HUBEC.

Keywords

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Human brain endothelial cells (HUBEC) promote SCID repopulating cell expansion through direct contact

Information for

Open access

Opportunities

Help and information

Human brain endothelial cells (HUBEC) promote SCID repopulating cell expansion through direct contact

Abstract

Reprints and Corporate Permissions

Academic Permissions

Related research

To cite this article:

Download citation

Information for

Open access

Opportunities

Help and information

Keep up to date

Your download is now in progress and you may close this window

Login or register to access this feature