The effect of acute dissociation on the electrophysiological properties of rat dorsal root ganglion neurons

Abstract

The acutely dissociated neurons from the dorsal root ganglia (DRGs) are extensively used. The effects of acute dissociation on the properties of these neurons are, however, not clear. In this study, the action potentials (APs) were recorded from both acutely dissociated and in vivo identified DRG neurons with patch clamp and sharp electrode recording techniques, respectively. We found that acute dissociation slowed both the depolarizing and repolarizing rate of APs, and elongated the AP duration (APD). The lower recording temperature presented in the acutely dissociated neurons contributed to about 10% of these differences. The major contributor of these differences was possibly modulation of the mRNA expression especially those of the ion channels, as suggested by our observation that acute dissociation significantly reduced the mRNA abundance of Nav1.6–1.9. In conclusion, acute dissociation altered the electrophysiological properties of the DRG neurons; the disrupted gene-expression pattern may contribute to this effect.

Keywords:

• Action potential
• acute dissociation
• dorsal root ganglion
• in vivo recording
• patch clamp
• real-time PCR

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work is supported by grants from National Nature Science Foundation of China [NSFC Grant Nos.: 81100818 and 30670854] sponsored to Dr Linlin Gao.