Flow Cytometry: General Concepts and Common Clinical Applications

Abstract

Flow cytometry is becoming an increasingly important diagnostic and research tool in the fields of immunology, hematology, and oncology. Because references to this technique are now commonplace in the medical literature, physicians and scientists should be familiar with the main features of the flow cytometer. This article explains the basic design of a flow cytometer and discusses two of its important functions, namely immunophenotyping and DNA analysis. A brief review of some specific clinical applications of flow cytometry is also presented.