Two-Dimensional Gel Electrophoresis of Proteins in Healthy Roots and Clubroots of Brassica oleracea

Abstract

Seedlings of Brassica oleracea were infected by the pathogenic fungus Plasmodiophora brassicae. causing clubroot formation. Proteins from healthy roots and clubroots were extracted by two different methods and analysed by two-dimensional gel electrophoresis. Host-proteins induced during clubroot infection were not analysed, since the fungus was always present in the infected roots. This work showed that several proteins present in non-infected roots were lost or strongly reduced in the infected roots.

Key Words:

• cabbage
• fungus
• infection
• Plasmodiophora brassicae
• protein extraction