Stable expression of a GFP-reporter gene in the red alga Cyanidioschyzon merolae

Satoru WatanabeDepartment of Bioscience, Tokyo University of Agriculture, Tokyo, Japan

Jun SatoDepartment of Bioscience, Tokyo University of Agriculture, Tokyo, Japan

Sousuke ImamuraChemical Resources Laboratory, Tokyo Institute of Technology, Yokohama, Japan;Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama, Japan

Mio OhnumaCollege of Science, Rikkyo University, Tokyo, Japan

Yusaku OhobaDepartment of Bioscience, Tokyo University of Agriculture, Tokyo, Japan

Taku ChibazakuraDepartment of Bioscience, Tokyo University of Agriculture, Tokyo, Japan

Kan TanakaChemical Resources Laboratory, Tokyo Institute of Technology, Yokohama, Japan;Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama, Japan

Hirofumi YoshikawaDepartment of Bioscience, Tokyo University of Agriculture, Tokyo, Japan;Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Saitama, JapanCorrespondence[email protected]

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Abstract

The unicellular red alga Cyanidioschyzon merolae is used as a model organism to investigate the basic architecture of photosynthetic eukaryotes. We established a stable expression system for the green fluorescent protein fused with the phycocyanin-associated rod linker (APCC) protein in C. merolae, which was clearly localized on the plastid. This system should be useful in the genetic engineering of C. merolae.

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Acknowledgments

We thank Ms Tomoko Kojima for critical reading of the manuscript. This study was supported by Grants-in-Aid 22681010, 24117521, and 25440129 (to S.I.), and 21370015, 23120505, and 2424806 (to K.T.) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan, and by a Sasakawa Scientific Research Grant from The Japan Science Society (to S.W.).

Notes

Abbreviations: GFP, green fluorescent protein; MA2 medium, modified Allen’s 2 medium.

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Stable expression of a GFP-reporter gene in the red alga Cyanidioschyzon merolae

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Stable expression of a GFP-reporter gene in the red alga Cyanidioschyzon merolae

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