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Abstract
A rapid protocol for polar lipid profiling was applied to Euglena gracilis lipid metabolism by LipidBlast, an MS/MS spectral similarity search tool. The similarity search results suggested anoxia-induced polar lipid metabolism in Euglena characterized by the accumulation of differential lipid classes, carbon chain lengths, and unsaturated bond numbers. The informatics-supported MS spectral search provides an alternative option for global lipid profiling studies.
Funding
Funding was provided by the Strategic International Cooperative Program (SICP) – Joint Research Type Japanese (JST) – US (NSF) Joint Research “Metabolomics for a low carbon society” (METABOLOMICS).
T.K. and O.F. were also supported by the US National Science Foundation (MCB 1139644) and the US National Institutes of Health grants (P20 HL113452 and U24 DK097154).
Notes
Abbreviations: LC, liquid chromatography; MS, mass spectrometry; FT-ICR/MS, Fourier transform ion cyclotron resonance mass spectrometry; MGF, Mascot Generic Format; LC-IT/TOFMS, liquid chromatography linear ion trap time-of-flight mass spectrometry; ESI, electrospray ionization; TIC, total ion chromatogram; lysoPE, lysoglycerophosphoethanolamine; PLA2, phospholipase A2; MGDG, monogalactosyldiacylglycerol.