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Abstract
The metabolism of quercetin was investigated in Mythimna separata larvae. Quercetin 4′-O-sulfate was mainly identified in the frass when 6th instar larvae were fed artificial diets containing 1% quercetin. In the case of the 3rd instar larvae, a larger amount of quercetin was detected in the frass. M. separata larvae had different metabolic strategies for quercetin at different developmental stages.
Funding
This study was partly supported by grant-aid for scientific research [grant number 22380068], [grant number 24120006] from the Ministry of Education, Culture, Sports, Science and Technology of Japan. TA was the recipient of a research fellowship for young scientists (No. 20.3494) from the Japan Society for the Promotion of Science.
Notes
Abbreviations: CDL, curved desolvation line; COE, carboxylesterases; DMSO, dimethyl sulfoxide; ESI, electron spray ionization; GSTs, glutathione S-transferases; LC/MS, liquid chromatography/mass spectrometry; NMR, nuclear magnetic resonance; P450s, cytochrome P450 monooxygenases; PAPS, 3′-phosphoadenosine 5′-phosphosulfate; TMS, trimethylsilane.