Abstract
The isolation of biosurfactant-producing yeasts from food materials was accomplished. By a combination of a new drop collapse method and thin-layer chromatography, 48 strains were selected as glycolipid biosurfactant producers from 347 strains, which were randomly isolated from various vegetables and fruits. Of the producers, 69% were obtained from vegetables of the Brassica family. Of the 48 producers, 15 strains gave relatively high yields of mannosylerythritol lipids (MELs), and were identified as Pseudozyma yeasts. These strains produced MELs from olive oil at yields ranging from 8.5 to 24.3 g/L. The best yield coefficient reached 0.49 g/g as to the carbon sources added. Accordingly, MEL producers were isolated at high efficiency from various vegetables and fruits, indicating that biosurfactant producers are widely present in foods. The present results should facilitate their application in the food and related industries.
Graphical Abstract
Mannosylerythritol lipids (MELs) producers were isolated from food materials by an improved drop collapse method and thin-layer chromatography.
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Acknowledgments
We thank Ms A. Sugimura, a fellow of the Japan Industrial Technology Association, for technical assistance. This study was partially supported by the Industrial Technology Research Grant Program (06A17501c) of the New Energy and Industrial Technology Development Organization (NEDO) of Japan.