![Sample our Bioscience journals, sign in here to start your access, Latest two full volumes FREE to you for 14 days]({"type":"image" , "src" : "/sda/5925/TOC-Subject-Sample-2021-Bioscience.jpg"})
Abstract
From the pellicle formed on top of brewing coconut water vinegar in Sri Lanka, three Acetobacter strains (SL13E-2, SL13E-3, and SL13E-4) that grow at 42 °C and four Gluconobacter strains (SL13-5, SL13-6, SL13-7, and SL13-8) grow at 37 °C were identified as Acetobacter pasteurianus and Gluconobacter frateurii, respectively. Acetic acid production by the isolated Acetobacter strains was examined. All three strains gave 4% acetic acid from 6% initial ethanol at 37 °C, and 2.5% acetic acid from 4% initial ethanol at 40 °C. Compared with the two other strains, SL13E-4 showed both slower growth and slower acetic acid production. As well as the thermotolerant SKU1108 strain, the activities of the alcohol dehydrogenase and the aldehyde dehydrogenase of SL13E-2 and SL13E-4 were more stable than those of the mesophilic strain. The isolated strains were used to produce coconut water vinegar at higher temperatures than typically used for vinegar production.
Graphical Abstract
From the pellicle formed on top of brewing coconut water vinegar in Sri Lanka, three Acetobacter pasteurianus strains and four Gluconobacter frateurii strains were isolated.
Acknowledgment
We wish to thank C.D. De Fonseka & Sons (Pvt) Ltd., Panadura, Sri Lanka, for providing coconut vinegar samples. We are grateful to Professor Kazunobu Matsushita of Yamaguchi University for providing A. pasteurianus SKU1108.
Notes
Abbreviations: AAB, acetic acid bacteria; ADH, alcohol dehydrogenase; ALDH, aldehyde dehydrogenase; ITS, internal transcribed spacer; TCA, tricarboxylic acid; v/v, volume/volume; w/v, weight/volume.
