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Abstract
Eight wild soybean accessions with different saponin phenotypes were used to examine saponin composition and relative saponin quantity in various tissues of mature seeds and two-week-old seedlings by LC–PDA/MS/MS. Saponin composition and content were varied according to tissues and accessions. The average total saponin concentration in 1 g mature dry seeds of wild soybean was 16.08 ± 3.13 μmol. In two-week-old seedlings, produced from 1 g mature seeds, it was 27.94 ± 6.52 μmol. Group A saponins were highly concentrated in seed hypocotyl (4.04 ± 0.71 μmol). High concentration of DDMP saponins (7.37 ± 5.22 μmol) and Sg-6 saponins (2.19 ± 0.59 μmol) was found in cotyledonary leaf. In seedlings, the amounts of group A and Sg-6 saponins reduced 2.3- and 1.3-folds, respectively, while DDMP + B + E saponins increased 2.5-fold than those of mature seeds. Our findings show that the group A and Sg-6 saponins in mature seeds were degraded and/or translocated by germination whereas DDMP saponins were newly synthesized.
Graphical Abstract
Germination down-regulates group A saponins and up-regulates DDMP and group B saponins
Acknowledgment
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0013600).
Notes
Abbreviations: ATSC, average total saponin composition; DDMP, 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one; LC–PDA/MS/MS, liquid chromatography–photodiode array detector/mass spectrometry/mass spectrometry; TLC, thin layer chromatography; SS-A, soyasapogenol A; SS-B, soyasapogenol B, SS-E, soyasapogenol E; SS-H, soyasapogenol H; SS-I, soyasapogenol I; SS-J, soyasapogenol J.
