Anti-obese and anti-diabetic effects of a mixture of daidzin and glycitin on C57BL/6J mice fed with a high-fat diet

Abstract

We investigated the effects of a mixture of daidzin and glycitin, which are the glycoside-form isoflavones of daidzein and glycitein, respectively, on body weight, lipid levels, diabetic markers, and metabolism in a high-fat diet (HF) fed C57BL/6J mice for 92 days. The mice were divided into basic diet group (CON), HF group, and HF companied with the isoflavone mixture group (HFISO). Results showed that mice in HFISO had a significantly lower body weight and adipose tissue compared to HF group. Blood glucose, serum HbA_1c, and serum insulin also showed lower levels in HFISO group. In addition, higher hepatic GSH level and lower serum 8-hydroxy-2′-deoxyguanosine (8-OHdG) level were found in HFISO group mice. This suggests that the regulation of oxidative stress by daidzin and glycitin was closely related to the suppression of adipose tissue and the progression of diabetes.

Graphical Abstract

Adipose cells enlargement occurs on high-fat diet feeding mice. The supplementary of isoflavones may regulate the oxidative stress, and suppress the adipose tissue and the progression of diabetes.

Key words:

• C57BL/6J mice
• daidzin and glycitin mixture
• obesity
• diabetes
• oxidative stress

Funding

Financial support for the present study was provided by the Scientific Research Foundation for Overseas scholars of Educational Commission of Heilongjiang Province of China [grant number 1253HQ0012]; the Initial Founding of Scientific Reasearch of Heilongjiang Bayi Agricultural University [grant number XYB2013-25]; and the National Natural Science Foundation [grant number 31171657].

Notes

Abbreviations: AUC, area under the curve; CPT, carnitine palmitoyl transferase; FAS, fatty acid synthase; HDL-Chol, high-density lipoprotein cholesterol; HbA1_c, hemoglobin A1_c; HMW adiponectin, high molecular weight adiponectin; NEFA, non-esterified fatty acids; OGTT, oral glucose tolerance test; T-BA, total bile acid; T-Chol, total-cholesterol; TG, triglyceride; TNF-α, tumor necrosis factor-α; PL, phospholipids; 8-OHdG, 8-hydroxy-2′-deoxyguanosine.

Author contribution: Animal experiments and manuscript preparation (YZ), providing sample and experimental places (KI), and data analysis and manuscript revision (CY).