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Abstract
Factors that increase protein thermostability are of considerable interest in both scientific and industrial fields. Disulfide bonds are one of such factors that increase thermostability, but are rarely found in intracellular proteins because of the reducing environment of the cytosol. Here, we report the first example of an intermolecular disulfide bond between heteromeric subunits of a novel-type phosphoserine phosphatase from a thermophilic bacterium Hydrogenobacter thermophilus, which contributes to the protein thermostability at the physiological temperature. Comparison of remaining soluble proteins between wild-type and cysteine-deleted mutant using SDS-PAGE revealed that the disulfide bond increases the thermostability of the whole protein by tightly connecting a subunit with low solubility to the partner with higher solubility. Furthermore, it was strongly suggested that the disulfide bond is formed and contributes to the stability in vivo. This finding will open new avenues for the design of proteins with increased thermostability.
Graphical abstract
Intermolecular disulfide bond was found in a heterodimeric protein from a thermophilic bacterium. It is essential for the protein thermostability.
Acknowledgments
The authors gratefully acknowledge Shoichiro Horita for discussion, and the help of Makoto Ato, Suhee Cho, and Masaru Ishizaki, who provided technical assistance with several molecular biology techniques.