Microbial production of poly(lactate-co-3-hydroxybutyrate) from hybrid Miscanthus-derived sugars

Jian SunDivision of Applied Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan

Camila UtsunomiaDivision of Applied Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan

Shohei SasakiDivision of Applied Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan

Ken’ichiro MatsumotoDivision of Applied Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan

Toshihiko YamadaField Science Center for Northern Biosphere, Hokkaido University, Sapporo, Japan

Toshihiko OoiDivision of Applied Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan;CREST, Kawaguchi, Japan

Seiichi TaguchiDivision of Applied Chemistry, Graduate School of Engineering, Hokkaido University, Sapporo, Japan;CREST, Kawaguchi, JapanCorrespondence[email protected]

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Abstract

P[(R)-lactate-co-(R)-3-hydroxybutyrate] [P(LA-co-3HB)] was produced in engineered Escherichia coli using lignocellulose-derived hydrolysates from Miscanthus × giganteus (hybrid Miscanthus) and rice straw. Hybrid Miscanthus-derived hydrolysate exhibited no negative effect on polymer production, LA fraction, and molecular weight of the polymer, whereas rice straw-derived hydrolysate reduced LA fraction. These results revealed that P(LA-co-3HB) was successfully produced from hybrid Miscanthus-derived sugars.

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Microbial production of poly(lactate-co-3-hydroxybutyrate) from hybrid Miscanthus-derived sugars

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Microbial production of poly(lactate-co-3-hydroxybutyrate) from hybrid Miscanthus-derived sugars

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