Effect of miR-143-3p on C2C12 myoblast differentiation

Abbreviations: MyoD, Myogenic differentiation; myf5, myogenic factor 5; ACTA1, Actin-α 1; MyoG, Myogenin; MRF4, Myogenic regulatory factor 4; MyHC, Myosin heavy chain; Wnt5a, Wingless-type family member 5a; LRP5, Low-density lipoprotein receptor-related protein 5; Axin2, Axis formation inhibitor 2; β-catenin, Beta-catenin.

Abstract

MicroRNAs are a class of 18–22 nucleotide non-coding RNAs that modulate gene expression by associating with the 3′ untranslated regions of mRNAs. A large number of microRNAs are involved in the regulation of myoblast differentiation, many of which remain undiscovered. In this study, we found that miR-143-3p was upregulated during C2C12 myoblast differentiation and over-expression of miR-143-3p significantly inhibited the relative expression levels of MyoD, MyoG, myf5, and MyHC genes, especially in the later stages of differentiation. In addition, miR-143-3p inhibited expression of genes involved in the endogenous Wnt signaling pathway during C2C12 myoblast differentiation, including Wnt5a, LRP5, Axin2, and β-catenin. These results indicate that miR-143-3p represents a new myogenic differentiation-associated microRNA that can inhibit C2C12 myoblast differentiation, especially in the later stages of differentiation.

Graphical abstract

MiR-143-3p may inhibit C2C12 myoblast differentiation by regulating marker genes related myoblast differentiation and Wnt signaling pathway.

Key words:

• miR-143-3p
• C2C12
• myoblast differentiation
• Wnt pathway

Notes

Abbreviations: MyoD, Myogenic differentiation; myf5, myogenic factor 5; ACTA1, Actin-α 1; MyoG, Myogenin; MRF4, Myogenic regulatory factor 4; MyHC, Myosin heavy chain; Wnt5a, Wingless-type family member 5a; LRP5, Low-density lipoprotein receptor-related protein 5; Axin2, Axis formation inhibitor 2; β-catenin, Beta-catenin.