A novel Ca²⁺-signal transduction inhibitor, kujigamberol C, isolated from Kuji amber

ABSTRACT

A novel labdane type diterpenoid, 15-nor-8-labden-13-ol, named kujigamberol C, was isolated from Kuji amber using a modified isolation method to increase the yield of biologically active compounds. The structure was determined using HREIMS, 1D and 2D NMR. Kujigamberol C showed growth-restoring activity against mutant yeast via Ca²⁺-signal transduction.

Graphical abstract

A novel Ca²⁺-signal transduction inhibitor, kujigamberol C, was isolated from Kuji amber. It showed the growth-restoring activity against the mutant yeast.

KEYWORDS:

• Kuji amber
• Saccharomyces cerevisiae
• 15-nor-8-labden-13-ol
• kujigamberol C

Author contributions

K.K. designed the research and supervised manuscript preparation. H.T., H.K., M.M., and H.S. performed the experiments. All authors reviewed the manuscript.

Acknowledgments

We are grateful to Ms. Shizuko Nakajo from the Center for Regional Collaboration in Research and Education of Iwate University for HREIMS; to Prof. Hisao Ando, Ibaraki University for personal communications about Kuji amber; and to Emeritus Professor Tokichi Miyakawa of Hiroshima University for providing the YNS17 strain. We would like to thank Editage (www.editage.jp) and Emeritus Professor Don R Phillips, La Trobe University for English language editing.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supplementary material

Supplemental data for this article can be accessed here.

Additional information

Funding

This work was partially supported by the New Energy and Industrial Technology Development Organization (NEDO: 1014005).